Beta-Alanine & Sodium Bicarbonate: A Winning Combo For Athletes?

Beta-alanine and sodium bicarbonate are some of the most popular ergogenic supplements (supplements intended to enhance physical performance, stamina, or recovery) on the market. Why? Most athletes assume that they actually work.

And it didn’t take long before supplement manufacturers started mixing the two. They weren’t just jocks in lab coats mixing random viles, eithermany scientists actually predicted a synergistic effect from mixing the two. 

But recent research suggests that not only does combining the two show no meaningful gain in athletic performance, but that both supplements on their own are ineffective. 

However, because of limitations in the study, I disagree. 

CONTRADICTING RESULTS ON EFFECT OF SODIUM BICARBONATE AND BETA-ALANINE 

Danaher, et al. (2014) found no effect of combining both during performance test with fixed intensity and volume. And if you look at the study design of the latest bicarbonate + beta-alanine cocktail (or BB, for short) study by scientists from the University of Sao Paulo (da Silva 2018), you again find that no meaningful performance gains occurred. 

Yet,  Tobias et al. (2013) tested upper body performance and didn't limit either intensity or volume and did see increased performance. Actually, the BB almost doubled the total work the subjects performed. 

So what gives? Is the BB cocktail a flop, or a win? 

A CLOSER LOOK AT THE STUDY ON SODIUM BICARBONATE AND BETA-ALANINE

Interestingly, it appears that da Silva’s study (which did not show any meaningful increase in performance) capped training intensity and total volume. 

Let’s take a closer look at the most recent study by da Silva 2018.  

The scientists had their reasons to standardize the workload. Without this standardization, the team wouldn't have been able to create an objective study. For example, scientists needed a guarantee that one group won't exercise 10 minutes longer or at 10% higher work rates than the other.

But let's (re-)address that later. For now, let’s take a closer look at the other key parameters of what I feel is a well-designed, interesting, and eventually highly educative study, despite (or because of) the lack of significant performance differences:

  • The subjects in da Silva 2018 (who found no meaningful gains) were 72 trained cyclists (5 ± 4 years of experience in cycling, 9 ± 3 h of training/week, consisting of 235 ± 92 km/week) with a VO2max of 59-60 ml/kg/min. 
    • What does this all mean? They were serious athletes. 
  • Beta-alanine was preloaded for a month at a comparatively high dose (28 days at 6.4g/d; two capsules were ingested four times daily vs. maltodextrin, (error in abstract) the placebo).
    • Participants preloaded the study with either a placebo or beta-alanine. 
  • The 0.3g/kg body weight of sodium bicarbonate (or calcium carbonate placebo) was supplemented within max. 5 minutes 60 minutes before the performance test in 1-g gelatin capsules under supervision to ensure full compliance. Gastrointestinal discomfort was reported prior to and 60 min using a 10-point scale - serial loading protocol as I've described it in previous articles about the bicarb was not used.
    • Essentially, that’s a boatload of baking soda to consume right before exercise! No wonder they had GI distress. 
  • The food intake during the supplementation period was 'monitored' via 3-day food diaries the subjects had to fill on 2 non-consecutive weekdays and 1 weekend day. The analyses of these food logs showed that the subjects consumed ~2200kcal/d with approximately 52%, 18.5%, and 26.5% of the energy coming from carbs, protein, and fats, respectively - without inter-group differences.
  • The actual testing sessions that were conducted after pre-test and familiarization sessions consisted of a high-intensity intermittent cycling protocol (4x 60s at 110% of the subjects' individual maximal power-output with 60s rests between bouts) that was followed by a 30-kJ time-trial performance test. Both tests performed twice - once before and once after the 28 days of beta-alanine or placebo supplementation (compare the illustration of the study design and main trials in Figure 1).
sodium bicarbonate

Figure 1: Experimental design (upper panel) and overview of the main trials (lower panel). BA β-alanine, SB sodium bicarbonate, PL placebo, CaCO3 placebo for sodium bicarbonate, lac plasma lactate analysis, gas blood gas analysis, HICT-110% high-intensity cycling test performed at 110% of Wmax (da Silva 2018)

As it is necessary for any double-blind, parallel-group, placebo-controlled trial, the participants were randomly allocated to the BA (β-alanine + placebo; n = 20, 3 drop-outs), SB (placebo + sodium bicarbonate; n = 20, 3 drop-outs), BASB (β-alanine + sodium bicarbonate; n = 20, 1 drop-out), or PLA (placebo + placebo; n = 20, 2 drop-outs) groups and asked to identify their group allocation (i.e., BA or maltodextrin and SB or calcium carbonate) to verify the blinding process.

Maltodextrin (the abstract falsely claims it was dextrose, which would be a very bad placebo due to its characteristic taste) and calcium carbonate were the placebos for BA and SB. Moreover, the randomisation was performed in blocks of 4 with groups being matched for time to complete a 30-kJ test to avoid significant baseline performance differences between the groups.

And finally, blood was sampled before and in-between exercise bouts and the time trial, respectively, and the VO2 consumption measured continuously before and during the HICT110% test.

That brings me to the first issue with the the study. 

CAN YOU DOUBLE-BLIND BETA-ALANINE AND SODIUM BICARBONATE?

Not really, because one gives you the tingles and the other...the runs. And trained athletes would certainly recognize these effects. 

The fact that three volunteers reported paresthesia (the tingles) with BA (BASB group) and nine participants (SB: 5; BASB: 4) reported gastrointestinal discomfort after ingesting sodium bicarbonate, confirms that blinding is not possible for the two agents.

Even though seven volunteers in the BA, five individuals in SB, six in BASB, and five in the PLA group rightly identified the arm (BA/PLA) they had been randomized to, the Fischer’s exact test showed a p-value of p = 0.85 for BA and p = 0.82 for SB, respectively. Apparently, it was a double-blind study in spite of tingling and tummy aches. 


baking soda and beta-alanine

Figure 2: Effects of β-alanine (BA) and sodium bicarbonate (SB) on the time to complete 30-kJ time-trial performance. The grey bar on c represents the 95% confidence interval of the coefficient of variation of the test. Positive and negative confidence intervals crossing zero on d were deemed nonsignificant (da Silva 2018).

Now, as previously announced, da Silva et al. did not find a significant effect of any of the three supplementation protocols on the subjects exercise performance. 

However, results did suggest non-significant performance improvements, with the largest effect size being and a borderline significant effect being observed in the BA + SB group.

What does all this mean? 

There was a measurable effect, with the most meaningful in the sodium bicarbonate and beta-alanine group, but no significant performance increases. 

WHY SODIUM BICARBONATE AND BETA-ALANINE DIDN’T GET A FAIR TRIAL 

If the increase in performance wasn’t significant, why would you care? 

I’m glad you asked. 

These results are not actually surprising when you consider the structure of the study.

You see, the benefits of the supplements depends on the build-up of sufficient amounts of H+ (good ol’ hydrogen) in a process called buffering. You can think of this chemical reaction like a sponge soaking up water—at a certain point, it is so saturated it can’t absorb any more liquid. 

Within the very short (~60s) bouts of exercise that was aborted when the subjects hit the 30-kJ of exercise mark, the H+ requirement probably wasn't met. Or with consideration of the non-significant benefits, it was at least not met to a sufficient degree.

sodium bicarbonate buffer

Figure 3: The amelioration of the decline in blood pH and bicarbonate shows that the SB treatment did what it was supposed to do - work as a buffer. However, as Figure 2 shows, the differences in pH and blood HCO3 were obviously too small to make a significant difference during the very short 30kJ performance test (da Silva 2018).

As Figure 3 shows, this doesn't mean that the buffers didn't work. 

As you can see, sodium bicarbonate successfully buffered the decrease in blood pH and we can assume that a muscle biopsy would have shown slightly reduced intra-cellular H+ levels due to beta-alanine. 

What this study really shows is that the biochemical differences to the control trials were too small and the inter-group variance too large to detect significant performance benefits.

beta alanine glycolytic

My plot of the effect sizes for the impact of beta-alanine (BA), sodium bicarbonate (SB), the combination of both (BASB), and placebo on the glycolytic energy contribution illustrates quite nicely that it's bicarbonate, not beta-alanine that will keep you going towards the end of a team sport event or in the sprint before the finish line.

IS THERE ANYTHING ELSE WORTH LOOKING AT IN THE STUDY?

It appears that sodium bicarbonatenot beta-alanineincreases the important glycolytic energy contribution during short bursts of high-intensity exercise. 

This is significant because it's often said that beta-alanine gives you an increased ability to tap into your glycogen stores that will provide the coveted “second wind.” 

Now, if we assume that the sodium bicarbonate advantage is not a result of bicarbonate messing with the indirect measurement of the glycolytic energy contribution (remember, no biopsies were performed), da Silva et al. suggest that absence of this benefit in beta-alanine may be due "to the total amount of H+ that can be neutralised by the working muscles" by sodium bicarbonate v. beta-alanine, respectively.

OTHER ISSUES WITH THE DA SILVA STUDY

There were two important limitations of the study the scientists willingly admit in the discussion of the results:

  • The first limitation is the lack of direct muscle analyses that could have (a) told us if and to what degree the muscle carnosine actually changed in response to the beta-alanine supplementation and (b) confirmed that the indirect measurement of the effects on energy metabolism was indeed accurate. 
  • The second limitation, which is not related to the study design, is a temporary malfunctioning of laboratory equipment which made it impossible for researchers "to undertake the estimation of the energy systems in all participants" (da Silva 2018). It’s possible the analysis of a complete dataset would have shown measurable and significant increases in glycolytic energy contribution for beta-alanine, as well.

The same must be assumed of the lactate buffering effects of sodium bicarbonate. In the scientists' multiple comparison analysis of the post-HICT-110% plasma lactate levels in the SB group, the latter approached significance (t = − 1.82, p = 0.074), and were significantly higher after the 30-kJ TT in both, the SB only (t = − 2.67, p = 0.01) and the BASB group (t = − 2.61, p = 0.011).

beta alanine research

Figure 4: Effects of β-alanine (BA) and sodium bicarbonate (SB) supplementation on the absolute contribution of the energy systems during the HCT-110% test (da Silva 2018). The increase glycolytic flux and improved ability to tap into the readily available glucose sources can be ascribed to the pH buffering effect of SB (Sutton 1981).


Unfortunately, lactate still has an unwarrantedly bad rep (Cairns 2006). It is thus important to note that increased lactate levels are a necessary consequence of an increased use of glucose and by no means a reason to expect performance decrements.

In fact, almost all sodium bicarbonate studies which tested the level of lactate in the subjects' blood found it to be significantly increased in the presence of significant performance benefits!

That's something people tend to forget in a day and age where even the superiority of glucose as the #1 substrate for high-intensity exercise is questioned (sorry keto fans, but that's simply what the contemporary evidence says).

THE TAKEAWAY: TO SUPPLEMENT, OR NOT TO SUPPLEMENT

While significant effects on the performance markers measured in the study at hand were not observed, da Silva et al. found non-significant increases in exercise performance in both tests, especially, when the two H+ buffers were combined, and a potentially highly relevant 20% increase in the subjects ability to tap into their glycogen stores in the SB and BASB groups.

In competitive sport, this difference may well make the difference between winning an Olympic medal and ending up with a disappointing fourth rank.

carnosine

Bodybuilders have a sign. elevated muscle carnosine levels, it is yet not clear if that's an adaptational response to prolonged repetitive exposure to low muscle pH, the consumption of high carnosine foods like chicken, supplement use, or the use of anabolic steroids (Tallon 2005). Other studies show that men store more carnosine than women (Mannion 1992), omnivores more than vegetarians (Harris 2007), and that genetic factors which have hitherto only been confirmed in our omnivore 'cousins', the pigs (D'Astous-Pagé 2017), may figure as well. It is thus hardly surprising that the BA research as such yielded highly variable results which are, just as it's the case for the study at hand, difficult to interpret if the changes in muscle carnosine aren't measured.


The statement that sodium bicarbonate and beta-alanine "don't work" should rather read “sodium bicarbonate and beta-alanine exert mea-surable, yet highly variable performance benefits.”

The effects may not have reached statistical significance because

(1) the efficacy of both agents has a high inter-individual variability, with beta-alanine potentially failing in subjects with diet or training related naturally high carnosine levels (cf. Figure 5) and sodium bicarbonate countering its own ergogenic effects via gastrointestinal distress (which was reported by nine participants in the study at hand), or because

(2) H+ buffers excel in exercise capacity tests, i.e. tests in which you exert yourselves to the point of volitional exhaustion, as opposed to performance tests with a fixed point like the 30 kJ test in the study at hand, or because

(3) the results of the study (despite being one of the better-powered investigations), may still "lack of statistical significance [...] due to insufficient statistical power to detect small effects" (my emphasis in da Silva 2018).

It's also possible that all three contributed synergistically to the lack of statistical significance of the results.

Instead of trying to make a general statement about the two ergogenics, which have also made the ISSN's TOP5 list of OTC ergogenic supplements, only recently (Kerksick 2018), we should use the publication of the study at hand to remind ourselves that the benefits of any supplement are specific to the sport, exercise, and even muscle group engaged. 

baking soda and endurance

Figure 6: With an upper-body Wingate test, the study by Tobias et al. (2013) I covered 5 years ago used didn't just use an exercise capacity test that didn't limit the total work done, but also tested the effects on a different muscle group - et voila: Tobias et al. found significant increases in total work and mean power with the combination of BA +SB.

So, what should you do? Well, one thing should be obvious:

Don’t throw away your 5kg bag of sodium bicarbonate and your 1kg tub of beta-alanine. Even though the performance benefits in the study at hand were at best borderline significant, there's a reason that both agents have been around for years (beta-alanine) and decades (bicarbonate).

In the absence of sufficient research to tell for sure who will and who won't benefit from using them, it’s best to make up your own mind on their effectiveness for you.  

About the author:

References:

  • Cairns, Simeon P. "Lactic acid and exercise performance." Sports Medicine 36.4 (2006): 279-291.
  • Carr, Benjamin M., et al. "Sodium bicarbonate supplementation improves hypertrophy-type resistance exercise performance." European journal of applied physiology 113.3 (2013): 743-752.
  • da Silva, Rafael Pires, et al. "Effects of β-alanine and sodium bicarbonate supplementation on the estimated energy system contribution during high-intensity intermittent exercise." Amino Acids (2018): 1-14.
  • Danaher, Jessica et al. "The effect of β-alanine and NaHCO3co-ingestion on buffering capacity and exercise performance with high-intensity exercise in healthy males." Eur J Appl Physiol (2014) 114:1715–1724.
  • Harris, Roger C., et al. "The carnosine content of V Lateralis in vegetarians and omnivores." (2007): A944-A944.
  • Kerksick, Chad M., et al. "ISSN exercise & sports nutrition review update: research & recommendations." Journal of the International Society of Sports Nutrition 15.1 (2018): 38.
  • Mannion, A. F., et al. "Carnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans." European journal of applied physiology and occupational physiology 64.1 (1992): 47-50.
  • D'Astous-Pagé, Joël, et al. "Identification of single nucleotide polymorphisms in carnosine-related genes and effects of genotypes on pork meat quality attributes." Meat science 134 (2017): 54-60.
  • Sutton, J. R., N. L. Jones, and C. J. Toews. "Effect of pH on muscle glycolysis during exercise." Clinical science 61.3 (1981): 331-338.
  • Tallon, Mark J., et al. "The carnosine content of vastus lateralis is elevated in resistance-trained bodybuilders." Journal of Strength and Conditioning Research 19.4 (2005): 725.
  • Tobias, Gabriel, et al. "Additive effects of beta-alanine and sodium bicarbonate on upper-body intermittent performance." Amino acids 45.2 (2013): 309-317.
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